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human breast cancer epithelial cell line mcf 7  (ATCC)


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    ATCC human breast cancer epithelial cell line mcf 7
    Human Breast Cancer Epithelial Cell Line Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38917 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast cancer epithelial cell line mcf 7/product/ATCC
    Average 99 stars, based on 38917 article reviews
    human breast cancer epithelial cell line mcf 7 - by Bioz Stars, 2026-02
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    human breast cancer epithelial cell line mcf 7  (ATCC)
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    ATCC human breast cancer epithelial cell line mcf 7
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    mcf7 human epithelial breast cancer cells  (ATCC)
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    human epithelial breast cancer cells mcf7  (ATCC)
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    ATCC human epithelial breast cancer cells mcf7
    (Ai) Immunoblots of LC3, LAMP1, p-mTOR and mTOR in whole cell lysates of MCF-7 and 4T1 treated with HAP (0.5 and 0.75 μg/ml in MCF-7 and 4T1 respectively) for 30 mins and thrombin (10 nmol/L for 3 hours). (Aii) Densitometric analysis of the protein expression of LC3II:LC3I, p-mTOR/mTOR and LAMP1 normalized against GAPDH in MCF-7 and against Cyclophilin B in 4T1. (Bi) Immunoblots of SQSTM1/p62 in the whole cell lysates of MCF-7 and 4T1, where <t>MCF7</t> cells treated with the synthetic peptides PFAISED (50 μM), SFLLRN (50 μM) and scramble peptide (HTPPPPFISEDASGY) and 4T1 cells with PFISED (75 μM), SFFLRN (75 μM) and scramble peptide (HTPPPPFISEDASGY) for 4 hours. (Bii) Quantitative analysis of the expression of SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Ci) Representative images of the immunoblots of the autophagy markers LC3, LAMP1 and SQSTM1/p62 in whole cell lysates of MCF-7 and 4T1 treated with different dosages (5, 25, 50, 75 and 100 μM) of both the peptides PFAISED and PFISED for 4 hours. (Cii) Densitometric analysis of the expression of LC3II:LC3I and LAMP1 & SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Di) Representative immunofluorescence images of MCF-7 and 4T1 cells. MCF-7 cells were treated with the peptide PFAISED (50 μM) and 4T1 cells were treated with the peptide PFISED (75 μM) for 4 hours and stained using dyes included in the Autophagy assay kit (ab139484) of Abcam and observed in a confocal microscope under FITC channel. (Dii) Quantification of the Mean fluorescence intensity of green fluorescence (FITC) per cell. (Ei) Representative immunoblots of different signalling molecules of autophagy including p-mTOR, mTOR, p-AMPK, AMPK, p-ULK1 (Ser757), p-ULK1 (Ser317), ULK-1 and Beclin1 in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Eii) Quantification of p-mTOR/mTOR, p-AMPK/AMPK, p-ULK1 (Ser757)/ULK1, P-ULK1 (Ser317)/ULK1 and Beclin1 normalized against GAPDH by densitometric analysis. (Fi) Representative immunoblots of the three MAP kinase molecules Erk1/2, p38 and Jnk and their phosphorylated forms in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Fii) Quantitative analysis of the expression of p-Erk1/2/ Erk, p-p38/p38 and p-Jnk/Jnk by densitometry.
    Human Epithelial Breast Cancer Cells Mcf7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell culture 101 human breast cancer epithelial cell line mcf 7  (ATCC)
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    ATCC cell culture 101 human breast cancer epithelial cell line mcf 7
    (Ai) Immunoblots of LC3, LAMP1, p-mTOR and mTOR in whole cell lysates of MCF-7 and 4T1 treated with HAP (0.5 and 0.75 μg/ml in MCF-7 and 4T1 respectively) for 30 mins and thrombin (10 nmol/L for 3 hours). (Aii) Densitometric analysis of the protein expression of LC3II:LC3I, p-mTOR/mTOR and LAMP1 normalized against GAPDH in MCF-7 and against Cyclophilin B in 4T1. (Bi) Immunoblots of SQSTM1/p62 in the whole cell lysates of MCF-7 and 4T1, where <t>MCF7</t> cells treated with the synthetic peptides PFAISED (50 μM), SFLLRN (50 μM) and scramble peptide (HTPPPPFISEDASGY) and 4T1 cells with PFISED (75 μM), SFFLRN (75 μM) and scramble peptide (HTPPPPFISEDASGY) for 4 hours. (Bii) Quantitative analysis of the expression of SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Ci) Representative images of the immunoblots of the autophagy markers LC3, LAMP1 and SQSTM1/p62 in whole cell lysates of MCF-7 and 4T1 treated with different dosages (5, 25, 50, 75 and 100 μM) of both the peptides PFAISED and PFISED for 4 hours. (Cii) Densitometric analysis of the expression of LC3II:LC3I and LAMP1 & SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Di) Representative immunofluorescence images of MCF-7 and 4T1 cells. MCF-7 cells were treated with the peptide PFAISED (50 μM) and 4T1 cells were treated with the peptide PFISED (75 μM) for 4 hours and stained using dyes included in the Autophagy assay kit (ab139484) of Abcam and observed in a confocal microscope under FITC channel. (Dii) Quantification of the Mean fluorescence intensity of green fluorescence (FITC) per cell. (Ei) Representative immunoblots of different signalling molecules of autophagy including p-mTOR, mTOR, p-AMPK, AMPK, p-ULK1 (Ser757), p-ULK1 (Ser317), ULK-1 and Beclin1 in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Eii) Quantification of p-mTOR/mTOR, p-AMPK/AMPK, p-ULK1 (Ser757)/ULK1, P-ULK1 (Ser317)/ULK1 and Beclin1 normalized against GAPDH by densitometric analysis. (Fi) Representative immunoblots of the three MAP kinase molecules Erk1/2, p38 and Jnk and their phosphorylated forms in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Fii) Quantitative analysis of the expression of p-Erk1/2/ Erk, p-p38/p38 and p-Jnk/Jnk by densitometry.
    Cell Culture 101 Human Breast Cancer Epithelial Cell Line Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human epithelial breast cancer cell line  (ATCC)
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    ATCC human epithelial breast cancer cell line
    (Ai) Immunoblots of LC3, LAMP1, p-mTOR and mTOR in whole cell lysates of MCF-7 and 4T1 treated with HAP (0.5 and 0.75 μg/ml in MCF-7 and 4T1 respectively) for 30 mins and thrombin (10 nmol/L for 3 hours). (Aii) Densitometric analysis of the protein expression of LC3II:LC3I, p-mTOR/mTOR and LAMP1 normalized against GAPDH in MCF-7 and against Cyclophilin B in 4T1. (Bi) Immunoblots of SQSTM1/p62 in the whole cell lysates of MCF-7 and 4T1, where <t>MCF7</t> cells treated with the synthetic peptides PFAISED (50 μM), SFLLRN (50 μM) and scramble peptide (HTPPPPFISEDASGY) and 4T1 cells with PFISED (75 μM), SFFLRN (75 μM) and scramble peptide (HTPPPPFISEDASGY) for 4 hours. (Bii) Quantitative analysis of the expression of SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Ci) Representative images of the immunoblots of the autophagy markers LC3, LAMP1 and SQSTM1/p62 in whole cell lysates of MCF-7 and 4T1 treated with different dosages (5, 25, 50, 75 and 100 μM) of both the peptides PFAISED and PFISED for 4 hours. (Cii) Densitometric analysis of the expression of LC3II:LC3I and LAMP1 & SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Di) Representative immunofluorescence images of MCF-7 and 4T1 cells. MCF-7 cells were treated with the peptide PFAISED (50 μM) and 4T1 cells were treated with the peptide PFISED (75 μM) for 4 hours and stained using dyes included in the Autophagy assay kit (ab139484) of Abcam and observed in a confocal microscope under FITC channel. (Dii) Quantification of the Mean fluorescence intensity of green fluorescence (FITC) per cell. (Ei) Representative immunoblots of different signalling molecules of autophagy including p-mTOR, mTOR, p-AMPK, AMPK, p-ULK1 (Ser757), p-ULK1 (Ser317), ULK-1 and Beclin1 in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Eii) Quantification of p-mTOR/mTOR, p-AMPK/AMPK, p-ULK1 (Ser757)/ULK1, P-ULK1 (Ser317)/ULK1 and Beclin1 normalized against GAPDH by densitometric analysis. (Fi) Representative immunoblots of the three MAP kinase molecules Erk1/2, p38 and Jnk and their phosphorylated forms in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Fii) Quantitative analysis of the expression of p-Erk1/2/ Erk, p-p38/p38 and p-Jnk/Jnk by densitometry.
    Human Epithelial Breast Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human epithelial breast cancer cell line/product/ATCC
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    erα human breast cancer epithelial cell lines mcf 7  (ATCC)
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    ATCC erα human breast cancer epithelial cell lines mcf 7
    (Ai) Immunoblots of LC3, LAMP1, p-mTOR and mTOR in whole cell lysates of MCF-7 and 4T1 treated with HAP (0.5 and 0.75 μg/ml in MCF-7 and 4T1 respectively) for 30 mins and thrombin (10 nmol/L for 3 hours). (Aii) Densitometric analysis of the protein expression of LC3II:LC3I, p-mTOR/mTOR and LAMP1 normalized against GAPDH in MCF-7 and against Cyclophilin B in 4T1. (Bi) Immunoblots of SQSTM1/p62 in the whole cell lysates of MCF-7 and 4T1, where <t>MCF7</t> cells treated with the synthetic peptides PFAISED (50 μM), SFLLRN (50 μM) and scramble peptide (HTPPPPFISEDASGY) and 4T1 cells with PFISED (75 μM), SFFLRN (75 μM) and scramble peptide (HTPPPPFISEDASGY) for 4 hours. (Bii) Quantitative analysis of the expression of SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Ci) Representative images of the immunoblots of the autophagy markers LC3, LAMP1 and SQSTM1/p62 in whole cell lysates of MCF-7 and 4T1 treated with different dosages (5, 25, 50, 75 and 100 μM) of both the peptides PFAISED and PFISED for 4 hours. (Cii) Densitometric analysis of the expression of LC3II:LC3I and LAMP1 & SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Di) Representative immunofluorescence images of MCF-7 and 4T1 cells. MCF-7 cells were treated with the peptide PFAISED (50 μM) and 4T1 cells were treated with the peptide PFISED (75 μM) for 4 hours and stained using dyes included in the Autophagy assay kit (ab139484) of Abcam and observed in a confocal microscope under FITC channel. (Dii) Quantification of the Mean fluorescence intensity of green fluorescence (FITC) per cell. (Ei) Representative immunoblots of different signalling molecules of autophagy including p-mTOR, mTOR, p-AMPK, AMPK, p-ULK1 (Ser757), p-ULK1 (Ser317), ULK-1 and Beclin1 in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Eii) Quantification of p-mTOR/mTOR, p-AMPK/AMPK, p-ULK1 (Ser757)/ULK1, P-ULK1 (Ser317)/ULK1 and Beclin1 normalized against GAPDH by densitometric analysis. (Fi) Representative immunoblots of the three MAP kinase molecules Erk1/2, p38 and Jnk and their phosphorylated forms in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Fii) Quantitative analysis of the expression of p-Erk1/2/ Erk, p-p38/p38 and p-Jnk/Jnk by densitometry.
    Erα Human Breast Cancer Epithelial Cell Lines Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erα human breast cancer epithelial cell lines mcf 7/product/ATCC
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    human mcf 7 breast cancer epithelial cells  (ATCC)
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    ATCC human mcf 7 breast cancer epithelial cells
    (Ai) Immunoblots of LC3, LAMP1, p-mTOR and mTOR in whole cell lysates of MCF-7 and 4T1 treated with HAP (0.5 and 0.75 μg/ml in MCF-7 and 4T1 respectively) for 30 mins and thrombin (10 nmol/L for 3 hours). (Aii) Densitometric analysis of the protein expression of LC3II:LC3I, p-mTOR/mTOR and LAMP1 normalized against GAPDH in MCF-7 and against Cyclophilin B in 4T1. (Bi) Immunoblots of SQSTM1/p62 in the whole cell lysates of MCF-7 and 4T1, where <t>MCF7</t> cells treated with the synthetic peptides PFAISED (50 μM), SFLLRN (50 μM) and scramble peptide (HTPPPPFISEDASGY) and 4T1 cells with PFISED (75 μM), SFFLRN (75 μM) and scramble peptide (HTPPPPFISEDASGY) for 4 hours. (Bii) Quantitative analysis of the expression of SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Ci) Representative images of the immunoblots of the autophagy markers LC3, LAMP1 and SQSTM1/p62 in whole cell lysates of MCF-7 and 4T1 treated with different dosages (5, 25, 50, 75 and 100 μM) of both the peptides PFAISED and PFISED for 4 hours. (Cii) Densitometric analysis of the expression of LC3II:LC3I and LAMP1 & SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Di) Representative immunofluorescence images of MCF-7 and 4T1 cells. MCF-7 cells were treated with the peptide PFAISED (50 μM) and 4T1 cells were treated with the peptide PFISED (75 μM) for 4 hours and stained using dyes included in the Autophagy assay kit (ab139484) of Abcam and observed in a confocal microscope under FITC channel. (Dii) Quantification of the Mean fluorescence intensity of green fluorescence (FITC) per cell. (Ei) Representative immunoblots of different signalling molecules of autophagy including p-mTOR, mTOR, p-AMPK, AMPK, p-ULK1 (Ser757), p-ULK1 (Ser317), ULK-1 and Beclin1 in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Eii) Quantification of p-mTOR/mTOR, p-AMPK/AMPK, p-ULK1 (Ser757)/ULK1, P-ULK1 (Ser317)/ULK1 and Beclin1 normalized against GAPDH by densitometric analysis. (Fi) Representative immunoblots of the three MAP kinase molecules Erk1/2, p38 and Jnk and their phosphorylated forms in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Fii) Quantitative analysis of the expression of p-Erk1/2/ Erk, p-p38/p38 and p-Jnk/Jnk by densitometry.
    Human Mcf 7 Breast Cancer Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mcf 7 breast cancer epithelial cells/product/ATCC
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    (Ai) Immunoblots of LC3, LAMP1, p-mTOR and mTOR in whole cell lysates of MCF-7 and 4T1 treated with HAP (0.5 and 0.75 μg/ml in MCF-7 and 4T1 respectively) for 30 mins and thrombin (10 nmol/L for 3 hours). (Aii) Densitometric analysis of the protein expression of LC3II:LC3I, p-mTOR/mTOR and LAMP1 normalized against GAPDH in MCF-7 and against Cyclophilin B in 4T1. (Bi) Immunoblots of SQSTM1/p62 in the whole cell lysates of MCF-7 and 4T1, where MCF7 cells treated with the synthetic peptides PFAISED (50 μM), SFLLRN (50 μM) and scramble peptide (HTPPPPFISEDASGY) and 4T1 cells with PFISED (75 μM), SFFLRN (75 μM) and scramble peptide (HTPPPPFISEDASGY) for 4 hours. (Bii) Quantitative analysis of the expression of SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Ci) Representative images of the immunoblots of the autophagy markers LC3, LAMP1 and SQSTM1/p62 in whole cell lysates of MCF-7 and 4T1 treated with different dosages (5, 25, 50, 75 and 100 μM) of both the peptides PFAISED and PFISED for 4 hours. (Cii) Densitometric analysis of the expression of LC3II:LC3I and LAMP1 & SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Di) Representative immunofluorescence images of MCF-7 and 4T1 cells. MCF-7 cells were treated with the peptide PFAISED (50 μM) and 4T1 cells were treated with the peptide PFISED (75 μM) for 4 hours and stained using dyes included in the Autophagy assay kit (ab139484) of Abcam and observed in a confocal microscope under FITC channel. (Dii) Quantification of the Mean fluorescence intensity of green fluorescence (FITC) per cell. (Ei) Representative immunoblots of different signalling molecules of autophagy including p-mTOR, mTOR, p-AMPK, AMPK, p-ULK1 (Ser757), p-ULK1 (Ser317), ULK-1 and Beclin1 in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Eii) Quantification of p-mTOR/mTOR, p-AMPK/AMPK, p-ULK1 (Ser757)/ULK1, P-ULK1 (Ser317)/ULK1 and Beclin1 normalized against GAPDH by densitometric analysis. (Fi) Representative immunoblots of the three MAP kinase molecules Erk1/2, p38 and Jnk and their phosphorylated forms in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Fii) Quantitative analysis of the expression of p-Erk1/2/ Erk, p-p38/p38 and p-Jnk/Jnk by densitometry.

    Journal: bioRxiv

    Article Title: Non-canonical activation of PAR1 induces autophagy in mammalian breast cancer cells

    doi: 10.1101/2025.01.11.632575

    Figure Lengend Snippet: (Ai) Immunoblots of LC3, LAMP1, p-mTOR and mTOR in whole cell lysates of MCF-7 and 4T1 treated with HAP (0.5 and 0.75 μg/ml in MCF-7 and 4T1 respectively) for 30 mins and thrombin (10 nmol/L for 3 hours). (Aii) Densitometric analysis of the protein expression of LC3II:LC3I, p-mTOR/mTOR and LAMP1 normalized against GAPDH in MCF-7 and against Cyclophilin B in 4T1. (Bi) Immunoblots of SQSTM1/p62 in the whole cell lysates of MCF-7 and 4T1, where MCF7 cells treated with the synthetic peptides PFAISED (50 μM), SFLLRN (50 μM) and scramble peptide (HTPPPPFISEDASGY) and 4T1 cells with PFISED (75 μM), SFFLRN (75 μM) and scramble peptide (HTPPPPFISEDASGY) for 4 hours. (Bii) Quantitative analysis of the expression of SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Ci) Representative images of the immunoblots of the autophagy markers LC3, LAMP1 and SQSTM1/p62 in whole cell lysates of MCF-7 and 4T1 treated with different dosages (5, 25, 50, 75 and 100 μM) of both the peptides PFAISED and PFISED for 4 hours. (Cii) Densitometric analysis of the expression of LC3II:LC3I and LAMP1 & SQSTM1/p62 normalized against GAPDH (in MCF-7) and Cyclophilin B (in 4T1). (Di) Representative immunofluorescence images of MCF-7 and 4T1 cells. MCF-7 cells were treated with the peptide PFAISED (50 μM) and 4T1 cells were treated with the peptide PFISED (75 μM) for 4 hours and stained using dyes included in the Autophagy assay kit (ab139484) of Abcam and observed in a confocal microscope under FITC channel. (Dii) Quantification of the Mean fluorescence intensity of green fluorescence (FITC) per cell. (Ei) Representative immunoblots of different signalling molecules of autophagy including p-mTOR, mTOR, p-AMPK, AMPK, p-ULK1 (Ser757), p-ULK1 (Ser317), ULK-1 and Beclin1 in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Eii) Quantification of p-mTOR/mTOR, p-AMPK/AMPK, p-ULK1 (Ser757)/ULK1, P-ULK1 (Ser317)/ULK1 and Beclin1 normalized against GAPDH by densitometric analysis. (Fi) Representative immunoblots of the three MAP kinase molecules Erk1/2, p38 and Jnk and their phosphorylated forms in the whole cell lysate of MCF-7 treated with different dosages of the peptide PFAISED, ranging from 5μM to 100 μM. (Fii) Quantitative analysis of the expression of p-Erk1/2/ Erk, p-p38/p38 and p-Jnk/Jnk by densitometry.

    Article Snippet: Human epithelial breast cancer cells MCF7 (ATCC, HTB-22), murine epithelial breast cancer cells 4T1 (ATCC, CRL-2539), and human normal breast epithelial cells MCF10A (ATCC, CRL-10317) were cultured (passages 10-12) and maintained in Minimum Essential Medium (MEM) (Gibco, 61100061), RPMI 1640 (Gibco, 23400021) and Mammary Epithelial Cell Basal Medium (MEBM) (Lonza, CC-3151) Mammary Epithelial Cell Growth Medium (MEGM) SingleQuots supplements (Lonza, CC-4136) respectively.

    Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Microscopy, Fluorescence